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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: Dev Biol. 2018 Dec 27;446(2):180–192. doi: 10.1016/j.ydbio.2018.11.023

Fig. 5. Tbx18:Cre and Tbx18:BAC-iCre expressing cells give rise to adult neurons and glia.

Fig. 5.

Cre-expressing lineages are marked by ZsGreen (Green) and nuclei are counter-stained with Hoechst (Blue). Tbx18:Cre and Tbx18:BAC-iCre strongly label lineages in the pelvic ganglia (A-D) as well as neuroendocrine cells (E, F) in the prostate epithelial compartment. Panels A-D include sub-panels marked with roman numerals that are the same section imaged with different wavelength channels. Tbx18:Cre does not label mature neurons in the pelvic ganglia, which are stained with the neuronal NeuN antibody (Aii), although these mature neurons are labeled by the Tbx18:BAC-iCre (Bii); white arrows. All cells marked in the pelvic ganglia by the Tbx18:Cre are satellite glia cells that wrap around the neurons, detected with marker S100 (Ci-iii). Tbx18:BAC-iCre also labels the satellite glia (Di-iii); white arrowheads. In addition, both Cre alleles mark neuroendocrine cells within the prostate epithelial compartment strongly (E-F), as confirmed by staining with an antibody to Serotonin (5-HT). Panels (Eii, Fii) are high resolution images crops of their corresponding panels (Ei, Fi) with arrowheads pointing to neuroendrocrine cells. Scale bars correspond to 50μm.