Skip to main content
. Author manuscript; available in PMC: 2019 Mar 3.
Published in final edited form as: Oncogene. 2018 Sep 3;38(6):838–851. doi: 10.1038/s41388-018-0462-2

Figure 7: Ate1 reduction drives metastasis of prostate orthotopic xenograft in mice.

Figure 7:

(A) Representative photo of luminescence detection of mouse tumors, 40 days after orthotopic injection of PC-3 and PC3-ML cells into the dorsal prostates of nude mice. The PC3 cells were transduced shRNA targeting Ate1 (shRNA #2) or NS control, and also transduced with luciferase as a tracing marker. PC3-ML, which has been shown to metastasize following orthotopic xenograft (27) was used as a positive control and transduced with NS control shRNA and luciferase. Arrows point to a few remote metastatic sites in these mice. (B) Quantification of primary tumor burden in these mice at the termination of the study was determined by bioluminescent signal count. No statistical significance was found. (C) Percentage of mice containing either only primary tumor, or with local invasion, or distal metastasis by these different cell lines is shown. Statistical significance was assessed by the Fisher’s exact test. *** = p<0.001. (D) Quantification of the average number of distal metastases per mouse. Error bars represent SEM. Statistical significance based on the Student’s t-test: **** = p<0.0001. See also Supp Fig. S7 for representative H&E stained images for the morphology of primary and metastatic tumors, as well as the distribution of the metastatic locations, formed by these above mentioned cells.