KEY RESOURCES TABLE
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Pax7; Species: Mouse IgG1 | Developmental Studies Hybridoma Bank | Cat# pax7, RRID:AB_528428 |
| Pan-Laminin; Species: Rabbit | Sigma-Aldrich | Cat# L9393 |
| Fluorescein; Species: Goat | Novus Biologicals | Cat# NB600–493 |
| FLAG M2; Species Mouse IgG1 | Sigma-Aldrich | Cat# F1804 |
| V5; Species: Mouse IgG2a | Thermo | Cat# R960–25 |
| Slug (C19G7); Species: Rabbit | Cell Signaling | Cat# 9585 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Fluoromount-G | SouthernBiotech | Cat# 0100–01 |
| Wheat Germ Agglutinin, Alexa Fluor™ 488 Conjugate | Thermo | W11261 |
| DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) | Thermo | D1306 |
| Experimental Models: Organisms/Strains | ||
| Gallus gallus | Sun State Ranch (Monrovia, CA, USA) | N/A |
| Oligonucleotides | ||
| Draxin morpholino w/ 3’Fluorescein: AAGGTGGAAGAAGCTGCCATAATCC | Hutchins and Bronner, 2018; GeneTools | N/A |
| Standard Control morpholino w/ 3’Fluorescein: CCTCTTACCTCAGTTACAATTTATA | GeneTools | N/A |
| Snail2 morpholino w/ 3’Fluorescein: TCTTGACCAGGAAGGAGC | Taneyhill et al., 2007; GeneTools | N/A |
| Nhel-V5-Snail2_F Primer: ATAGCTAGCGCCACCATGGGTAAGCCTATCCCTAAC | This paper; IDT | N/A |
| Xbal-V5-Snail2_R Primer: ACCTCTAGATCAGTGTGCTACGCAGCAG | This paper; IDT | N/A |
| Recombinant DNA | ||
| pCI-H2B-RFP | Betancur et al., 2010 | N/A |
| Draxin-FLAG | Hutchins and Bronner, 2018 | N/A |
| NC1.1 M3:eGFP | Simoes-Costa et al., 2012 | N/A |
| pCIG-V5-Snail2-IRES-nls-GFP | Liu et al., 2013 | Addgene Plasmid #44282 |
| pCIG | Megason and McMahon, 2002 | N/A |
| NC1.1M3-Snail2 | This paper | N/A |
| Software and Algorithms | ||
| ImageJ64 | NIH | N/A |
| Fiji | Schindelin et al., 2012 | N/A |
| Zen 2 Blue | Zeiss | N/A |
| Photoshop CC | Adobe | N/A |