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Mechanism of action. Double reciprocal plots for inhibition of Sts-1HP by the tetracycline derivative doxycycline (A) and the azo dye Congo red (B). For each, three concentrations of the inhibitor were used (shown as inset on each graph) and a linear fit was used. The lines fit to the data intersect the X-axis at a value close to zero, in both cases, indicating a competitive mode of inhibition.
