Table 3.
Active sulfonated azo dyes identified in 20K compound screen
| Name | Mol. Weight (g/mol) | Primary IC50 (μM) | Counterscreen IC50 (μM) | Promiscuity Indexa | Ki (μM) |
|---|---|---|---|---|---|
| Congo Red | 696.7 | 0.92 | >14.5 | 11 of 73 | 0.079 ± 0.02 |
| Chicago Sky Blue | 992.8 | 1.0 | >14.5 | 8 of 67 | NDb |
| NF-279 | 1401.1 | 2.4 | >29 | 5 of 75 | ND |
| Evans Blue | 960.8 | 3.1 | 23.6 | 19 of 82 | 0.39 ± 0.11 |
| PPNDS | 694.4 | 6.9 | >29 | 3 of 75 | ND |
a
The promiscuity index is the number of times this compound appeared as a hit in a screen compared to the number of total screens conducted that contained this compound – a lower number is desirable. This metric measures promiscuity reflected in all screens carried out by the Scripps Research Molecular Screening Center. Additional promiscuity measures can be seen in Tables S1 and S2.
b
ND: Not determined