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. 2019 Jan 4;71(1):165–180. doi: 10.1007/s10616-018-0274-9

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Development stages of Menyanthes trifoliata in vitro and in vivo (soil-grown) conditions. a Seeds after surface sterilization. b Seeds germination after 1 month, c 6 weeks old in vitro plant, d 3 month old plant in soil, eM. trifoliata after 1 year, f plants in vitro cultured in liquid SH medium (bar = 1 cm)