. 2019 Jan 4;71(1):165–180. doi: 10.1007/s10616-018-0274-9

Table 1.

The contents of secondary metabolites in Menyanthes trifoliata plants obtained in vitro and in vivo cultures

No.	Compounds	MtRV	MtAPV	MtRS	MtAPS
No.	Compounds	µg g⁻¹ dry weight
1	Betulinic acid	5437.15 ± 141.33 ^c	395.31 ± 14.5 ^a	3938.95 ± 82.65 ^b	390.00 ± 15.87 ^a
2	Vanillic acid	2.83 ± 0.16^c	2.23 ± 0.09^b	1.98 ± 0.09^b	1.43 ± 0.06^a
3	Syringic acid	113.80 ± 0.80 ^c	n.d.	29.96 ± 0.05 ^b	0.98 ± 0.02^a
4	Ferulic acid	13.80 ± 0.80^b	n.d.	5.09 ± 0.14^a	n.d.
5	Sinapinic acid	146.53 ± 7.03 ^d	71.16 ± 3.34^b	86.12 ± 5.81 ^c	45.84 ± 1.60^a
6	o-coumaric acid	3.42 ± 0.11^c	2.95 ± 0.13^c	2.07 ± 0.09^b	1.18 ± 0.03^a
7	Salicylic acid	8.68 ± 0.47^d	4.21 ± 0.26^b	5.10 ± 0.27^c	2.71 ± 0.12^a
8	Ellagic acid	518.11 ± 26.46 ^c	450.65 ± 14.22 ^b	326.98 ± 20.92 ^a	298.64 ± 12.54 ^a
9	Coumarin	17.69 ± 0.51^d	11.37 ± 0.62^c	5.45 ± 0.30^a	7.01 ± 37.2^b
10	t-3-hydroxycinnaminic acid	4.77 ± 0.25^d	2.03 ± 0.08^c	0.27 ± 0.008^a	1.43 ± 0.05^b
11	Hesperidin	60.73 ± 3.27^c	70.50 ± 3.80^d	39.17 ± 3.40^a	47.59 ± 2.28^b
12	t-cinnaminic acid	3.98 ± 0.21^c	3.11 ± 0.12^b	2.87 ± 0.07^b	1.89 ± 0.06^a
13	Hesperetin	43.04 ± 1.54^c	20.19 ± 0.78^b	20.94 ± 0.85^b	11.96 ± 0.56^a
14	Neochlorogenic acid	11.62 ± 0.66^c	11.87 ± 0.52^c	5.70 ± 0.19^a	7.73 ± 0.40^b
15	Gentisinic acid	11.65 ± 0.33^b	35.72 ± 1.28^d	7.64 ± 0.34^a	25.46 ± 1.19^c
16	Chlorogenic acid	177.34 ± 9.57 ^b	257.9 ± 14.70 ^c	128.77 ± 7.21 ^a	178.13 ± 7.83 ^b
17	Caffeic acid	1.16 ± 0.03^c	1.31 ± 0.02^c	0.65 ± 0.28^a	0.84 ± 0.02^b
18	1,3-dicaffeoylquinic acid	0.89 ± 0.02^d	0.22 ± 0.0072^b	0.35 ± 0.0042^c	0.15 ± 0.0075^a
19	p-Coumaric acid	10.74 ± 0.44^b	15.78 ± 0.42^c	6.89 ± 0.15^a	9.66 ± 0.41^b
20	Luteolin	9.08 ± 0.28^d	7.16 ± 0.27^c	5.74 ± 0.29^b	1.68 ± 0.06^a
21	Rutin	256.20 ± 3.24 ^d	152.99 ± 6.42 ^b	180.04 ± 2.86 ^c	82.34 ± 3.04 ^a
Sum of phenolic compounds		1416,06	1121,35	861,78	726,65

The compounds were determined in 80% aqueous methanol extracts from roots and aerial parts of plants obtained from in vitro (MtRV and MtAPV) and in vivo (MtRS and MtAPS) cultures. Different superscript letter within the rows indicate significant differences in the mean values at p < 0.05. The compounds present in the highest concentrations are given in bold