Table 2.
Summary of results of cell cultures in different media
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MSCs | MSCs | SCs | MSCs | SCs | SCs | MSCs | HEK | HEK | MSCs | SCs | MSCs | N/A | SCs | |
| SF | RS | SF | SF | S | SF | S | S | S | RS | SF | S | S | S | |
| X | X | X | XF | X | X | X | X | X | X | X | X | X | XF | |
| +/− | – | + | + | – | ? | – | ? | ? | – | + | – | – | + | |
| HKCSCs | + | – | +/− | x | – | – | – | x | x | – | ++ | – | – | +++ |
| 786-O vhl mut | – | – | – | – | x | – | x | x | x | x | + | x | – | ++ |
| 769-P vhl mut | x | – | x | + | – | x | – | x | x | – | x | – | – | +/− |
| ACHN vhl wt | – | – | x | +/− | – | – | – | x | x | – | ++ | – | – | x |
| Caki-2 vhl wt | x | – | x | +/− | – | – | – | x | x | – | – | – | – | x |
HEK medium for HEK cells, S medium contains serum, SF serum free medium, RS reduced serum medium, X xenogeneic medium, XF xeno-free medium
+ Visible 3D structures of diameter > 300 μm, suggesting tumor-like hypoxia inside the structure
+/− Visible small 3D structures or both 2D and 3D cell culture
– No visible 3D structures visible, but cells growing (i.e. in 2D or in small microaggregates)
X lack of cell growth in general