Fig. 6.

Determination of bile acid production in cell culture supernatants by LC/MS–MS with data of concentrations present in cell culture medium. Concentration of individual bile acids present in cell culture medium supplemented with FBS and amount of each bile acid (in μmol/L) after 7 days of cultivation are shown. The bile acids measured were: Taurocholic acid (TCA), taurochenodeoxycholic acid (TCDCA), taurodeoxycholic acid (TDCA) and taurolithocholic acid (TLCA), glycocholic acid (GCA), cholic acid (CA), glycochen- odeoxycholic acid (GCDCA), chenodeoxycholic acid (CDCA), glycodeoxycholic acid (GDCA), deoxycholic acid (DCA), glycolithocholic acid (GLCA), lithocholic acid (LCA). Data are presented as column scatter plots showing medians and interquartile ranges