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Cytotoxicity of TTO towards A-375 and HEp-2 tumor cells as determined by MTT assay. After 24 h, the estimated IC50 values were 0.038 ± 0.011% (v/v) for A-375 cells and 0.024 ± 0.017% (v/v) for HEp-2 cells using non-linear estimation method (GraphPad Prism 8 software, La Jolla, CA, USA, test version)
