Fig. 3.

Flow cytometry results of Annexin V/PI assays. A-375 and HEp-2 cells were either untreated control) or treated with their TTO IC₅₀ for 24 h. The lower left area contains cells negative for both Annexin V and PI (viable); cells in the lower right are positive for Annexin V but not PI (early apoptotic); cells in the upper left are Annexin V− and PI+ (late apoptotic); and cells in the upper right are both Annexin V+ and PI+ (necrotic)