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. 2019 Jan 1;71(1):461–473. doi: 10.1007/s10616-018-0287-4

Table 1.

Primers sequences used for amplification of P53, BAX, BCL-2, and the internal control β-actin

Gene Primer sequences Size of PCR product (bp)
P53 Forward: 5′-TCA GAT CCT AGC GTC GAG CCC-3′ 438
Reverse: 5′-GGG TGT GGA ATC AAC CCA CAG-3′
BAX Forward: 5′-ATG GAC GGG TCC GGG GAG CA-3′ 322
Reverse: 5′-CCC AGT TGA AGT TGC CGT CA-3′
BCL-2 Forward: 5′-GTG AAC TGG GGG AGG ATT GT-3′ 216
Reverse: 5′-GGA GAA ATC AAA CAG AGG CC-3′
β-actin Forward: 5′-CTG TCT GGC GGC ACC ACC AT-3′ 253
Reverse: 5′-GCA ACT AAG TCA TAG TCC GC-3′