Fig. 6.

Two-color imaging of mossy fibers and GABAergic interneurons in DG. (a) Mossy fibers in the hilus area expressing EGFP. The endogenous EGFP fluorescence was enhanced by antibody staining against EGFP, the secondary antibody was conjugated to Alexa 488 (green). Parvalbumin staining identified GABAergic interneurons shown in red (Video 5, MP4, 65 MB [URL: https://doi.org/10.1117/1.NPh.6.1.015005.5]). 1500 optical slices were acquired with a step size of $0.3\mu \mathrm{m}$, the shown data were deconvolved. Volume size $456\times 945\times 390\mu {\mathrm{m}}^3$. (b) Side view of the data shown in (a). (c) Segmented parvalbumin cells and mossy fibers reconstructed in 3-D. Magnification of the ROI marked in (b), showing connection between the cells.