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. 2019 Feb 8;9:1680. doi: 10.1038/s41598-018-38298-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HSPCs frequency in CHD patients. Mild coronary stenosis <50% (A) and severe coronary stenosis ≥70% (B), as detected by coronary angiography. (C) The percentage of CD11b+ cells in freshly isolated HSPCs (C). (D) HSPCs were plated on Methylcelluose cultures for 14 days. Cells were harvested and stained with anti-human CD11b. The percentage of CD11b+ cells was shown. (E) HSPCs frequency in subjects with and without CHD. (F) HSPCs frequency in CAD with different extents of stenosis. HSPCs frequencies in e and fare expressed as mean and 95% CI. (G–I) Representative FACS plot of HSPCs analysis. Among all samples, at least8728 (95%CI, 5783–11674) CD34+ cells were obtained in the analysis. (J,K) The correlation curves of HSPCs frequency and white blood cell count or neutrophil count in CHD patients, respectively.