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. 2019 Feb 8;9:1677. doi: 10.1038/s41598-019-39149-7

Table 1.

Sampling depth, richness and alpha diversity indices for haemolymph sequence libraries of juvenile P. ornatus.

Sample ID Lobster no. Sampling depth Richness estimators Diversity indices
Raw sequences Filtered sequences Obs. OTUs Good’s coverage (%) Phylum Class Order Family Genus Chao1 ACE Shannon Simpson
28 °C day 0
Culture-independent samples
28C0d1 1 7,532 4,354 109 97.5 6 11 19 18 21 144 145 2.54 0.81
28C0d2 2 4,790 2,660 119 95.5 7 10 16 16 15 130 136 3.71 0.96
28C0d3 3 4,248 2,389 160 93.3 6 12 16 14 18 177 181 4.39 0.98
28C0d4 4 9,229 6,979 230 96.7 8 13 25 30 32 320 280 4.19 0.95
28C0d5 5 9,689 6,978 274 96.1 11 19 32 35 39 344 367 4.33 0.96
28C0d6 6 4,972 3,755 237 93.7 13 19 31 30 34 281 318 4.26 0.97
28 °C day 6
Culture-independent samples
28C6d1 1 5,132 3,745 172 95.4 7 11 22 24 21 198 204 3.76 0.93
28C6d2 2 8,588 6,261 137 97.8 5 9 13 14 16 154 158 4.16 0.98
28C6d3 3 8,911 5,439 158 97.1 6 9 15 16 11 206 197 4.11 0.97
28C6d4 4 4,264 3,149 156 95.0 8 10 20 21 27 176 179 4.15 0.97
28C6d5 5 4,869 2,325 145 93.8 9 12 16 22 29 161 158 4.08 0.97
28C6d6 6 9,043 6,169 108 98.2 4 7 13 15 19 145 138 3.61 0.95
Culture-dependent samples
28C6d128a 1–6 12,306 10,051 140 98.6 2 3 6 4 11 155 159 2.56 0.85
34 °C day 6
Culture-independent samples
34C6d1 1 3,810 2,678 108 96.0 3 6 11 13 18 121 128 3.62 0.96
34C6d2 2 10,382 8,576 156 98.2 5 11 20 16 26 225 193 3.92 0.96
34C6d3 3 10,046 5,464 77 98.6 5 9 15 18 21 104 107 1.73 0.61
34C6d4 4 9,007 5,516 112 98.0 5 9 13 14 7 144 136 3.71 0.95
34C6d5 5 8,872 5,524 112 98.0 6 9 12 11 10 132 137 3.58 0.95
34C6d6 6 5,092 3,107 133 95.7 7 11 20 22 23 151 151 3.84 0.96
Culture-dependent samples
34C6d178a 1–6 12,012 9,064 197 97.8 2 4 6 7 14 203 205 3.66 0.94

aPool of all colonies cultured on marine agar.