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. 2019 Feb 8;9:1714. doi: 10.1038/s41598-018-37497-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Brain, acute demyelinating encephalitis with vacuolation of the white matter due to myelin sheath oedema. Acidophilic viral intranuclear inclusion in a shrunken neuron (black arrow head). Perivascular inflammatory changes are minimal with few cells (black arrows). HE staining. Bar = 100 µm. (B) Brain, strong staining of CDV antigen in neurons and in endothelial cells of a blood vessel (black arrow). IHC. Bar = 100 µm.