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Average melting temperatures (Tm) in the PCR-HRM of the positives control of the three Plasmodium species.
| Plasmodium species | Tm ± Sd (°C) Primer Endmal18sF-R | ANOVA | Tm ± Sd (°C) Primer Amzmal18sF-R | ANOVA |
|---|---|---|---|---|
| Plasmodium falciparum | 77,38 ± 0,04 | p < 0.0001* | 73,69 ± 0,02 | p ≪ 0.0001* |
| Plasmodium vivax | 77,72 ± 0,02 | 75,50 ± 0,01 | ||
| Plasmodium malariae | 78,55 ± 0,02 | 71,09 ± 0,01 |
Sd: Standard deviation; Tm: Melting Temperature; *ANOVA, *Significant difference among means (p < 0.05).
