Table 2.
Identification of Plasmodium spp. in human clinical and Anopheles vector samples by PCR-HRM.
| PCR-HRM | Snounou’s Nested PCR | Blood smears | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pv | Pf | Pm | Mx | Neg | Pv | Pf | Pm | Mx | Neg | Pv | Pf | Pm | Mx | Neg | |
| Human | 14 | 17 | 3 | 3 | 4 | 13 | 15 | 2 | 1 | 10 | 10 | 15 | 0 | 1 | 15 |
| An. darlingi | 1 | 1 | 0 | 0 | 98 | 0 | 0 | 0 | 0 | 100 | — | — | — | — | — |
| An. mattogrossensis | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | — | — | — | — | — |
| Anopheles spp. | 0 | 0 | 0 | 0 | 234 | 0 | 0 | 0 | 0 | 234 | — | — | — | — | — |
The Endmal18sF-R and Amzmal18sF-R primers were used to evaluate human samples (N = 41) and samples from Anopheles spp. (N = 342) collected in an area endemic with high malaria transmission in Amazonas, Colombia. PCR-HRM: Total detection using both primer pairs. Pv: P. vivax. Pf: P. falciparum. Pm: P. malariae. Mx: Mixed infection Pv/Pm only detected with Amzmal18sF-R primers. Neg: No detection.