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. 2019 Feb 8;10:687. doi: 10.1038/s41467-019-08604-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Cytokine and chemokine mRNA in ITC. a Distribution of IFNG transcript levels across all samples. b Sum of expression levels (tpm) for 37 cytokine and chemokine genes across all samples, per cell type. tpm, N = 6; Boxplots are described in Methods. Histogram overlays are normalized to mode. c Representative flow cytometry plots for IFN-γ intracellular staining 2 h after activation in the presence of cyclohexamide to block transcription or actinomycin D to block translation. d As in c, with MFI shown for N = 3, error is s.e.m., P-value is paired t test