Table 3.

Experimental matrix for the 2^6-2 design factors and responses for cytotoxicity.

Runs	Factors						Cytotoxicity* (R2)
	A	B	C	D	E	F
1	−1	−1	−1	1	−1	1	10.12 ± 6.88
2	−1	1	1	−1	−1	−1	31.29 ± 4.70
3	−1	−1	−1	−1	−1	−1	2.17 ± 0.66
4	−1	1	1	1	−1	1	18.76 ± 7.7
5	−1	−1	1	−1	1	1	17.71 ± 7.76
6	0	0	0	0	0	0	24.78 ± 3.15
7	1	1	−1	1	1	1	24.62 ± 7.75
8	1	−1	−1	−1	1	−1	29.20 ± 6.92
9	1	−1	1	−1	−1	1	17.15 ± 1.62
10	−1	−1	1	1	1	−1	42.24 ± 3.68
11	1	1	−1	−1	−1	−1	18.69 ± 2.92
12	1	−1	1	1	−1	1	67.33 ± 5.30
13	−1	1	−1	1	1	1	19.55 ± 3.06
14	1	1	1	−1	1	1	28.81 ± 2.79
15	0	0	0	0	0	0	23.10 ± 2.82
16	1	1	−1	1	−1	−1	12.78 ± 3.98
17	−1	1	−1	−1	1	1	3.87 ± 1.76
18	1	1	1	1	1	1	11.78 ± 3.06

A = pressure, B = temperature, C = processing time, D = material, E = sonication time and F = stirring rate.

*% of cell death of SCC25 at a concentration equivalent to 100 mg original leaf material /mL cell culture medium (mean ± SEM) (n = 3).