Table 5.

Estimated effects and coefficient for the processing cytotoxicity (R² = 99.96%).

Term	Effect	Coefficient	SE-coefficient	T	P
Constant		22.255	0.2977	74.74	0.009
A	8.079	4.039	0.2977	13.57	0.047*
B	−8.127	−4.063	0.2977	−13.65	0.047*
C	14.263	7.131	0.2977	23.95	0.027*
D	7.286	3.643	0.2977	12.23	0.052*
E	−0.064	−0.032	0.2977	−0.11	0.932
F	−13.832	−6.916	0.2977	−23.23	0.027*
AB	−8.433	−4.217	0.2977	−14.16	0.045*
AC	−4.314	−2.157	0.2977	−7.24	0.087
AD	−1.621	−0.811	0.2977	−2.72	0.224
AE	−5.322	−2.661	0.2977	−8.94	0.071
AF	−2.634	−1.317	0.2977	−4.42	0.142
BD	−12.232	−6.116	0.2977	−20.54	0.031*
BF	4.003	2.001	0.2977	6.72	0.094
ABD	−4.902	−2.451	0.2977	−8.23	0.077
ABF	6.908	3.454	0.2977	11.60	0.055
Centre points		1.685	0.8932	1.89	0.310

A = pressure, B = temperature, C = processing time, D = material, E atsonication time and F onstirring rate. SE = standard error. Statistically significant factors (ANOVA, P-value ≤ 0.05).