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. 2019 Feb 8;9:1718. doi: 10.1038/s41598-018-37222-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gβ subunit specificity to α_2a adrenergic receptors. Quantification of Gβ subunits interacting with α_2aARs in both adrenergic and non-adrenergic neurons (N = 4 unless otherwise noted on the graph with parentheses). Gβ subunits detected (fmol) from quantitative measurements were normalized by the amount of protein (mg), calculated using the volume and the protein concentration of precleared lysate used in co-IPs. We included several controls: unstimulated WT (WT no epi), HA-α_2aAR (HA-α_2aAR no epi), and stimulated WT (WT + epi) samples are all controls for the key sample, the Gβ and γ isoforms interacting with HA-α_2aAR. Gβ₂ and Gβ₄ specifically interact with activated α_2aARs present in all synaptic terminals. Data were presented as mean ± SEM and compared by a one-way ANOVA, **P < 0.01. Post hoc analysis was performed with Tukey’s multiple comparison test.