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. 2019 Feb 8;10(2):112. doi: 10.1038/s41419-019-1390-y

Fig. 3. miR-1260b negatively regulated SOCS6 by directly binding to its 3′-UTR.

Fig. 3

a The candidate gene targets were predicted by intersecting outputs from four distinct prediction algorithms (TargetScan, miRDB, RNA22, and microRNA.org). b The potential miR-1260b seed region at the 3′-UTR of SOCS6 mRNA was computationally predicted. SPCA1 cells were co-transfected with miR-1260b mimics (or NC) with pGL3-SOCS6 (or pGL3-SOCS6-mut) vector. Luciferase activity was normalized by the ratio of firefly and Renilla luciferase signals. 1: pGL3-SOCS6; 2: pGL3-SOCS6 + miR-1260b mimics; 3: pGL3-SOCS6 + NC; 4: pGL3-SOCS6 mut; 5: pGL3-SOCS6 mut + miR-1260b mimics; 6: pGL3-SOCS6 mut + NC. c SOCS6 mRNA in 120 NSCLC tissues and paired adjacent tissues was investigated by qRT-PCR. d IHC staining against SOCS6 assay was used to detect the effects of miR-1260b expression alteration on cell proliferation in the tissue samples. Magnification: × 200 and × 400. Scale bar is 100 μm and 50 μm. e There was an inverse correlation between miR-1260b and SOCS6. f, g A negative regulatory effect of miR-1260b on SOCS6 was tested by qRT-PCR and western blot. GAPDH was used as an internal control. The data expressed as the mean ± SD (*P < 0.05; **P < 0.01; ***P < 0.001)