Table 1.
Rhizosphere soil | Microorganism | Heat treatment | ||||
---|---|---|---|---|---|---|
No treatment | 40 °C | 60 °C | 80 °C | 121 °C | ||
Welsh onion | Bacteria | |||||
Gram-negative | 0.7–1.3 × 108 | 0.6–1.1 × 108 | <2.8 × 106 | ND | ND | |
Gram-positive | 0.7–1.0 × 108 | 5.0–9.7 × 107 | 2.0–3.9 × 107 | 1.9–3.5 × 107 | ND | |
Fungi | 0.8–3.1 × 106 | 0.5–2.4 × 106 | 0.3–1.8 × 104 | <1.5 × 102 | ND | |
Onion | Bacteria | |||||
Gram-negative | 3.4–6.0 × 107 | 2.6–4.7 × 107 | <1.9 × 106 | ND | ND | |
Gram-positive | 4.0–6.7 × 107 | 2.1–3.0 × 107 | 2.0–3.0 × 107 | 2.3–2.7 × 107 | ND | |
Fungi | 2.4–2.7 × 105 | 1.5–1.8 × 105 | 2.3–3.7 × 103 | <2.3 × 102 | ND |
The culturable bacterial densities were estimated using 1/10 strength tryptic soy agar, and Gram reaction was determined using the KOH method. The culturable fungal densities were estimated using rose bengal-streptomycin agar. The experiment was repeated three times. ND: not detected.