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. 2019 Feb 8;9:1715. doi: 10.1038/s41598-018-37559-7

Table 1.

Effect of heat treatment on population densities of culturable bacteria and fungi in Allium plant-cultivated soils (cfu gram−1 dry soil).

Rhizosphere soil Microorganism Heat treatment
No treatment 40 °C 60 °C 80 °C 121 °C
Welsh onion Bacteria
   Gram-negative 0.7–1.3 × 108 0.6–1.1 × 108 <2.8 × 106 ND ND
   Gram-positive 0.7–1.0 × 108 5.0–9.7 × 107 2.0–3.9 × 107 1.9–3.5 × 107 ND
Fungi 0.8–3.1 × 106 0.5–2.4 × 106 0.3–1.8 × 104 <1.5 × 102 ND
Onion Bacteria
   Gram-negative 3.4–6.0 × 107 2.6–4.7 × 107 <1.9 × 106 ND ND
   Gram-positive 4.0–6.7 × 107 2.1–3.0 × 107 2.0–3.0 × 107 2.3–2.7 × 107 ND
Fungi 2.4–2.7 × 105 1.5–1.8 × 105 2.3–3.7 × 103 <2.3 × 102 ND

The culturable bacterial densities were estimated using 1/10 strength tryptic soy agar, and Gram reaction was determined using the KOH method. The culturable fungal densities were estimated using rose bengal-streptomycin agar. The experiment was repeated three times. ND: not detected.