Fig. 5. Flow cytometric analysis of changes in cell populations in ldlr−/− mice treated with free celastrol or Cel-MC.

(a) Heatmap of fold change in cell populations. Each row represents an immune cell population, each column represents the organ from which the cells were isolated. Heatmap is on a log₂ scale, with yellow representing a fold increase and blue representing a fold decrease in that cell population, compared to the Blank MC control. Cell population as a percent of all immune cells for a given population in a given organ are also provided for: (b) aortic neutrophils, (c) aortic NK cells, (d) blood monocytes, and (e) blood neutrophils. All significant p-values are displayed on their graphs, calculated using Dunn’s multiple comparisons test. Cells were identified as follows: B cells – CD45⁺ CD19⁺, NK cells – CD45⁺ NK1.1⁺, T cells – CD45⁺ CD3⁺, Neutrophils – CD45⁺ Ly-6G⁺, Macrophages – CD45⁺ CD3^- NK1.1^- CD19^- Ly-6G^- F4/80⁺, Dendritic cells – CD45⁺ CD3^- NK1.1^- CD19^- Ly-6G^- F4/80^- CD11c⁺, Monocytes – CD45⁺ CD3^- NK1.1^- CD19^- Ly-6G^- F4/80^- CD11c^- CD11b⁺ Ly-6C⁺.