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. Author manuscript; available in PMC: 2019 Feb 9.
Published in final edited form as: Cancer Cell. 2018 Mar 12;33(3):435–449.e6. doi: 10.1016/j.ccell.2018.02.006

Figure 7: BCL-XL is a potential therapeutic target downstream of NRL.

Figure 7:

(A-E) Rescue experiments were performed in D458MED (A,B,D) and HDMB03 (A,C,E) MB cells. Cells were co-infected by lentivirus encoding control shRNA (Ctl), shRNA targeting NRL (shNRL#1 and #2) together with either a control vector (empty vector) or a BCL-XL-encoding retrovirus (BCL-XL). The level of the different proteins was analyzed by immunoblot (NS: non specific)(A). Cell viability (B-C) and the level of apoptosis quantified by measuring cleaved caspase 3 by FACS (D-E) were assessed. The p value was determined by unpaired t test. (F) Immunoblot conducted with the indicated antibodies on D458MED cells transduced with control shRNA (Ctl) or shRNAs targeting BCL-XL (shBCL-XL#1and #2). (G) Cell viability of D458MED cells transduced with lentivirus encoding control shRNA (Ctl, black line), or shRNA targeting BCL-XL (shBCL-XL#1 and shBCL-XL#2, in dark blue dashes and line). (H) The percentage of apoptosis was assessed in these different cultures by measuring cleaved caspase 3 levels by FACS. (I-J) Control and BCL-XL-targeted D458MED (I) or PDX2 (J) cells were orthotopically engrafted into the cerebellum of nude mice. Kaplan Meier survival curves for these mice are shown: Ctl: shRNA control (black line), shBCL-XL#1 and shBCL-XL#2 (dark blue dashes and line). The p value for survival was determined by log-rank (Mantel-Cox) test. **p-value<0.001. Bars represent the mean ± SD.