Table 2.
Time-dependent effect of MNP-lectin conjugates on sperm motility characteristics
| Other motion | Incubation time, min | P-values | |||
|---|---|---|---|---|---|
| parameters | 0 (N=18) |
30 (N=19) |
90 (N=15) |
120 (N=16) |
|
| VAP, μm/s | 70 ± 2a | 77.6 ± 2.1b | 73.2 ± 3.1c | 74.8 ± 1.7bc | < 0.0001 |
| VSL, μm/s | 35.4 ± 1.1a | 40.5 ± 0.8b | 45.1 ± 0.7c | 42.7 ± 1.3d | < 0.0001 |
| VCL, μm/s | 154.5 ± 4.1a | 164.7 ± 4.3b | 150.3 ± 4.8a | 160.4 ± 2.9b | 0.001 |
| ALH, μm | 8.3 ± 0.1a | 7.7 ± 0.1b | 7.2 ± 0.3c | 7.9 ± 0.1b | < 0.0001 |
| BCF, Hz | 38.1 ± 0.4a | 36.2 ± 0.5b | 37.9 ± 0.9a | 34.1 ± 0.6c | < 0.0001 |
| STR, % | 51.1 ± 0.6a | 54.2 ± 0.9b | 63.1 ± 2.5c | 58.1 ± 1.0d | < 0.0001 |
| LIN, % | 24.3 ± 0.5a | 26.9 ± 0.5b | 33.1 ± 2.2c | 28.2 ± 0.6b | < 0.0001 |
The table represents various sperm kinematic parameters, such as average path velocity (VAP), straight-line velocity (VSL), curve-linear velocity (VCL), head lateral amplitude (ALH), beat flagellum frequency (BCF), straightness (STR – VSL/VAP × 100), linearity (LIN – VSL/VCL × 100). Data are mean (±sem) of N observations of at least 4 independent replicates using pooled semen doses. Different letters on the same line denote significant differences (P<0.05)