Table 4.
Viability assessment of nanoselected spermatozoa by Flow cytometry
| Control1 | Nanoselected2 | |
|---|---|---|
| Reactive oxygen species | 1,069 ± 200 | 864 ± 200 |
| Plasma membrane integrity | 11.9 ± 25.6 | 8.6 ± 25.6 |
| Mitochondrial integrity | 426 ± 200 | 898 ± 224 |
1Non selected (Control) vs. 2double-selected (annexin V and lectin; Nanoselected) spermatozoa. Data are relative fluorescence intensities (mean±sem) of spermatozoa stained with H2-DCFDA (ROS production), propidium iodide (plasma membrane integrity), or JC-1 (mitochondrial potential). Fluorescence intensity values are proportional to the extent of damages of 4 independent replicates (mean±sem). Data within the same line are not significantly different (P>0.05)