Table 1.
Primers and probes used for RT-qPCR
| Primer or Probe | Gene | Sequence (5′- > 3′) or Assay ID |
|---|---|---|
| Primer | FUS | F: GCCAAGATCAATCCTCCATGAGTAGTG |
| R: TCCACGGTCCTGCTGTCCATAG | ||
| circ_002136 | F: CTTTCCGAGACATTTGCTGG | |
| R: CATGGAGATCACAATAAGGAACTC | ||
| P:FAM + TCTTCTTCTCCTCTGTCTTCC+MGB | ||
| GAPDH | F: GGACCTGACCTGCCGTCTAG | |
| R: TAGCCCAGGATGCCCTTGAG | ||
| P:FAM + CCTCCGACGCCTGCTTCACCACCT+Eclipse | ||
| CDK11A | F: AGAGGAAGAGGAGGAGGAGGAGAC | |
| R: CGAACCGTGACTCTGGAACAACC | ||
| SOX13 | F: CTGGACTTCAACCGAAATTTGA | |
| R: GTTCCTTCCTAGAAACCTCTCC | ||
| SPON2 | F: GATTGTAGACAGCGCCTCAGTTCC | |
| R: GACGCACTCAGCCTCTTCTTCG | ||
| Probe | MiR-138-5p U6 | 002284(Applied Biosystems) |
| 001973(Applied Biosystems) |