Table 3.
Primers used for ChIP experiments
| Gene | Binding site or Control | Sequence (5′- > 3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| SPON2 | PCR1 | F:TTTACCGAGTGCTAGAGCCG | 165 | 59.6 |
| R: AGGCTGCTGTGGCTGTTT | ||||
| PCR2 | F: CTTACGACGCAGGGTCTGG | 237 | 59.8 | |
| R:CGGCTCTAGCACTCGGTAAA | ||||
| PCR3 | F:ACCCAAGAAAATCAGCCAAAGC | 211 | 60.2 | |
| R:TCACTGTGGAATCGCGTGAG | ||||
| PCR4 | F:GTGCCCAGCATCTATTCTGGT | 207 | 59.9 | |
| R: CTACAGCGTCCCACAGACC | ||||
| FUS | PCR1 | F:AGTGTTTTGCAGTTACAAGACCTG | 97 | 59.8 |
| R:GGAAAGTGAGACTCAGAGACCC | ||||
| PCR2 | F: CGTCTTGGCTCACTGCAACT | 167 | 60.5 | |
| R:GTCAGGAGTTCGAGACCAGC |