Table 1.

Experimental designs and the results of the Box–Behnken Design

Std	Run	Factors				Responses
		A-glucose (g/L)	B-glycerol (mL/L)	C-tryptone (g/L)	D-yeast extract (g/L)	OD600	Conversion efficiency (%)
22	1	10	30	10	0	8.36	1.41
14	2	10	30	0	10	10.25	0.17
9	3	0	15	10	0	4.37	12.54
5	4	10	15	0	0	5.64	1.37
23	5	10	0	10	20	10.91	1.11
8	6	10	15	20	20	10.91	3.26
2	7	20	0	10	10	12.04	0.19
28	8	10	15	10	10	11.44	0.00
4	9	20	30	10	10	10.43	0.03
13	10	10	0	0	10	10.81	0.58
29	11	10	15	10	10	12.50	0.00
26	12	10	15	10	10	9.74	0.00
11	13	0	15	10	20	6.25	22.96
12	14	20	15	10	20	13.29	0.45
1	15	0	0	10	10	7.28	3.13
21	16	10	0	10	0	9.51	0.03
25	17	10	15	10	10	8.65	0.01
16	18	10	30	20	10	12.66	0.31
3	19	0	30	10	10	6.61	13.42
27	20	10	15	10	10	10.62	0.00
10	21	20	15	10	0	8.76	0.02
17	22	0	15	0	10	8.50	20.25
19	23	0	15	20	10	6.05	13.58
18	24	20	15	0	10	9.54	0.11
7	25	10	15	0	20	10.21	0.29
15	26	10	0	20	10	10.92	1.02
6	27	10	15	20	0	8.40	0.55
20	28	20	15	20	10	11.76	0.27
24	29	10	30	10	20	10.64	0.38

The highest responses of OD₆₀₀ and conversion efficiency were highlighted in italics