Table 3.

Results of agitation fermentation coupled with static process

No.	Time agitation process-static process	Tube volume (mL)	TUDCA (g/L)	T-7-KLCA (g/L)	TCDCA (g/L)	Ratio of TUDCA to TCDCA	TUDCA conversion efficiency (%)
1	0 h–0 h	▲	0.00	0.00	1.10	0.00	0.00
2	3.5 h–0 h	▲	0.04	0.00	1.06	0.04	3.55
3	6.5 h–0 h	▲	0.07	0.01	1.00	0.07	6.05
4	16.5 h–0 h	▲	0.18	0.04	0.89	0.20	15.94
5	0 h–16.5 h	2	0.15	0.02	0.93	0.16	13.23
6		15	0.27	0.04	0.79	0.34	24.24
7		50	0.26	0.01	0.83	0.31	23.30
8	0 h–22 h	2	0.19	0.05	0.86	0.22	17.22
9		15	0.30	0.03	0.78	0.40	27.73
10		50	0.28	0.06	0.76	0.36	25.21
11	0 h–48 h	2	0.19	0.05	0.86	0.22	17.01
12		15	0.30	0.06	0.74	0.41	27.22
13		50	0.21	0.16	0.73	0.28	18.92
14	3.5 h–13 h	2	0.54	0.03	0.52	1.05	49.58
15		15	0.56	0.04	0.50	1.11	50.76
16		50	0.58	0.04	0.48	1.20	52.70
17	3.5 h–18.5 h	2	0.54	0.06	0.49	1.10	49.49
18		15	0.53	0.07	0.50	1.05	47.94
19		50	0.52	0.07	0.51	1.02	47.10
20	3.5 h–44.5 h	2	0.50	0.09	0.51	0.98	45.52
21		15	0.51	0.09	0.50	1.02	46.42
22		50	0.51	0.10	0.49	1.05	46.60
23	6.5 h–10 h	2	0.52	0.07	0.51	1.02	47.41
24		15	0.51	0.06	0.52	0.98	46.74
25		50	0.52	0.06	0.52	1.00	47.20
26	6.5 h–15.5 h	2	0.42	0.07	0.61	0.69	38.25
27		15	0.41	0.08	0.61	0.67	37.36
28		50	0.43	0.06	0.60	0.71	39.25
29	6.5 h–41.5 h	2	0.50	0.09	0.51	0.96	45.19
30		15	0.49	0.09	0.52	0.94	44.38
31		50	0.51	0.09	0.50	1.03	46.66
32	16.5 h–5.5 h	2	0.19	0.06	0.85	0.22	17.25
33		15	0.20	0.07	0.83	0.24	17.83
34		50	0.24	0.09	0.76	0.32	22.28
35	16.5 h–31.5 h	2	0.22	0.09	0.80	0.27	19.62
36		15	0.32	0.09	0.69	0.47	29.21
37		50	0.32	0.09	0.69	0.46	28.94

▲These samples were directly taken from fermenters without static process. TUDCA conversion efficiency above 45.00% was highlighted in italics