Fig. 3.

TVA induces apoptosis in NPC cells through Akt and Bad inactivation. a. The expression of Akt, p-Akt, Bad, p-Bad and Mcl-1 in 5-8F cells was analyzed by western blot assay after the cells were treated with TVA. b. The expression of Akt, p-Akt, Bad, p-Bad and Mcl-1 in CNE-2 cells was analyzed by western blot assay after the cells were treated with TVA. Protein expression was quantified by normalization to the level of β-tubulin. c. Effects of IGF-1 on TVA-induced apoptosis as detected by an annexin V-FITC/PI staining assay. The number of apoptotic cells was determined after treatment with TVA (100 μM) in the presence or absence of IGF-1 (50 ng/mL) for 24 h. The values represent the means±standard deviation of three independent experiments. *P<0.05 and **P<0.01 versus the control group