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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2018 Aug 15;126:350–357. doi: 10.1016/j.freeradbiomed.2018.08.014

Fig. 3. CSE downregulates GPx3 and induces oxidative stress.

Fig. 3.

NHBE cells were treated with air or 10% CSE for 6 h. Following treatment; (A) Whole cell extracts were obtained and subjected to Western blotting for GPx3, COX2 and NOX4. β-Actin served as a loading control. (B) GPx3 activity was assessed in NHBE cells, as described in Materials and Methods. (C and D) Level of intracellular oxidative stress was examined in NHBE cells by fluorescence-based assays of ROS (C) and H2O2 (D) production. At least two independent experiments were performed. Data are expressed as the mean ± SD with n = 3; **P < 0.01, ***P < 0.001.