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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2018 Aug 15;126:350–357. doi: 10.1016/j.freeradbiomed.2018.08.014

Fig. 5. CSE-induced downregulation of GPx3 correlates with PPARγ expression/activity.

Fig. 5.

(A)NHBE cells, transfected with PPARγ-specific siRNA or scrambled siRNA (negative control), were treated with air (negative control) or 10% CSE for 6 h. Following treatment, whole cell extracts were obtained and subjected to Western blotting for PPARγ and GPx3. (B) NHBE cells, treated with the PPARγ antagonist GW9662 (1 μM) or vehicle control, were exposed to air (negative control) or 10% CSE for 6 h. Following treatment, whole cell extracts were obtained and subjected to Western blotting for PPARγ and GPx3. (C) NHBE cells, treated with 1 μM rosiglitazone or vehicle control, were exposed to air (negative control) or 10% CSE for 6 h. Following treatment, whole cell extracts were obtained and subjected to Western blotting for PPARγ and GPx3. In all the experiments, β-Actin served as a loading control. The results were reproduced independently at least two times. A representative image is shown.