Table 1.
Category | Stage in kinetoplast cycle | Expected set of abnormal trypanosomes |
---|---|---|
Class I | kDNA synthesis | 1KS1N, KS/KS1N, KS/KS2N |
Class II | Selection of Scission Site | KL/KS1N, KL/KS2N, 1KL1N, 1KS1N |
Class III | Scission of kDNA network | 1KU1N, 1KU2N, 0K1N. Electron microscopy shows one uncleaved kDNA per kinetoplast |
Class IV | Separation of Cleaved kDNA networks | 1KU1N, 1KU2N, 0K1N. Two cleaved kDNAs per kinetoplast, detected in electron microscopy analysis |
Class V | Partitioning of kinetoplasts into new cells | 2K1N, 0K1N |
Different types of kinetoplasts and the expected sets appearing together are presented for mutants belonging to classes I through V. Kinetoplast designations are as follows; K, kDNA containing one genomes’ worth of minicircles and maxicircles; KS, kinetoplast contains less < 0.5 of one genome’s equivalent of kDNA; KL, kinetoplast contains > 0.5 < 1 of one genome’s equivalent of kDNA; KU, kDNA has two genome’s amount of minicircles and maxicircles.