Table 2.
The effects of mutations in minCDE, clpP and lexA on cellular size and shape1
| Strain | lexA | clpP | minCDE | Average Cell Area | %Cells (Area>300) | Bumps | Mini cells |
|---|---|---|---|---|---|---|---|
| SS7117 | + | + | + | 160 | 1 | 0 | 0 |
| SS9190 | 51 | Δ | + | 330 | 28 | 0.04 | 0.19 |
| SS12699 | + | + | Δ | 570 | 75 | 0.02 | 2.06 |
| SS9194 | 51 | Δ | Δ | 840 | 70 | 0.14 | 2.06 |
1
850–3200 cells were counted for each strain. Average cell area is given in number of pixels. Bumps and mini-cells are given as a frequency per total number of cells. Cells were grown as mentioned the Experimental Procedures. The average cell area of ΔclpP lexA51 (SS9190) is significantly different (p < 0.001) from the ΔminCDE single mutant (SS12699) by the Student’s t-test. The differences in average cell area between both the ΔclpP lexA51(SS9190) double mutant and the ΔminCDE single mutant (SS12699) and the ΔclpP lexA51 ΔminCDE (SS9194) triple mutant are significant (p < 0.001).