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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Mol Microbiol. 2018 Dec 5;111(2):405–422. doi: 10.1111/mmi.14162

Table 5.

ΔclpP lexA51 recN-dependence of long/mini-cell and bumps phenotype in ΔsulA sulB+ (ftsZ+) strains with a BW25113 background1

Strain lexA clpP recNp recN Average Cell Area %Cells (Area>200) Bumps Mini cells
SS10044 + + + + 100 0 0 0
SS10047 51 + + + 140 8 0 0.02
SS10352 + Δ + + 100 0 0 0
SS12698 + + + Δ 100 0 0 0
SS10064 51 Δ + + 270 50 0.01 0.43
SS10354 + + op + 100 0 0 0
SS10355 + Δ op + 160 15 0.02 0.05
1

The strains used in this table are all derivatives of BW25113. They have a ΔsulA mutation instead of a sulB103 to prevent SOS filamentation. 2300–3700 cells were counted for each strain. Average Cell Area is given in number of pixels. Bumps and mini-cells are given as a frequency per total number of cells. Cells were grown in as mentioned the Experimental Procedures. All statistical tests were done using the Student’s t-test. The difference in average cell area of ΔclpP lexA51 (SS10064) and either of the single mutants (SS10047 and SS10352) is significant (p < 0.001). Likewise, the difference in average cell area of recNop ΔclpP (SS10355) and either of the single mutants (SS10354 and SS10352) is significant (p < 0.001).