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. Author manuscript; available in PMC: 2019 Feb 10.
Published in final edited form as: Mol Plant. 2017 Sep 28;10(11):1400–1416. doi: 10.1016/j.molp.2017.09.012

Figure 2. Constitutive Presence of ER Bodies in the ceh1 Is SA Independent.

Figure 2.

(A) Representative confocal images of WT, the SA-deficient mutant eds16, ceh1, and ceh1/eds16 leaves depicting constitutive presence of otherwise wound-inducible ER bodies independently of SA in ceh1 mutant backgrounds. White arrows show the ER body. Bars, 5 μm.

(B) Relative expression levels of ER marker genes in the aforementioned genotypes. Total RNA extracted from these genotypes was subjected to real-time qPCR analysis. The transcript levels were normalized to At4g26410 (M3E9) measured in the same samples. Data are the mean fold difference ± SD of three biological replicates each with three technical repeats. Different letters represent statistically significant differences (p < 0.05).

(C) Normalized iTRAQ protein abundance ratios of detected ER marker proteins in mutants (eds16, ceh1, and ceh1/eds16) relative to the WT plants. Data are means of n = 3 ± SEM. Single and double asterisks denote a statistically significant difference relative to WT and ceh1, respectively (p < 0.05) as determined by t-tests.