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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Brain Struct Funct. 2018 Oct 3;224(1):99–110. doi: 10.1007/s00429-018-1766-2

Figure 5: Chemoactivation of MCH neurons increase REM sleep in both MCH-Cre and MCH-Vglut2KO mice.

Figure 5:

Percentages of wake, NREMs and REMs in 3 h bins after intraperitoneal administration of vehicle (saline) or CNO (0.3 mg/kg, i.p., 10:00 AM) in MCH-Cre (n=10) and MCH-Vglut2KO (n=11) that were injected with AAV-hM3Dq into the lateral hypothalamus. Two-way ANOVA between gene and treatment (1–3 h: interaction F(1,31)=0.0069, p=0.93; treatment F(1,31)=28.65, p<0.0001; gene F(1, 31)=8.98, p=0.0053. 4–6 h: interaction F(1,31)=1.30; treatment F(1,31)=10.00, p=0.0035; gene F(1,31)=2.80, p=0.10) followed by Sidak’s Multiple Comparison test; * p<0.05, ** p<0.01. All data are mean ± SEM.