Fig. 3. Nucleoprotein (NP)-specific CD8 T-cell responses measured by the tetramers. rAd/B-NP(I) (A, B) and rAd/B-NP(V) (C, D) vaccinated mice were challenged with 5LD₅₀ doses of Influenza B/Shangdong or B/Yamagata. Lung cells were harvested 5 days after challenge, and stained with two types of D^d/NP_166–174 tetramer (V or I), anti-CD8, and anti-CD44 antibodies. (A, C) Representative dot plots of tetramer staining. The upper right quadrant area shows tetramer⁺CD44⁺ cells. (B, D) The percentages of tetramer-positive cells calculated among each group. ^a)p<0.01.