Table 1.
Bacterial strains and plasmids used in the present study.
| Strain or plasmid | Description | Source |
|---|---|---|
| STRAINS | ||
| S340 | S. Choleraesuis | (20) |
| S984 | S. Choleraesuis Δfur | This work |
| P. multocida 0818 | Wild-type P. multocida serotype A strain. Capsulated and virulent | (21) |
| S985 | P. multocida 0818 Δfur::kanR | This work |
| χ7232 | E. coli K-12, endA1 hsdR17 (rK-, mK+) glnV44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 λpir deoR (φ80dlac Δ(lacZ)M15) | (22) |
| χ7213 | E. coli K-12, thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4-2-Tc::Mu λpir ΔasdA4 Δzhf-2::Tn10 | (22) |
| PLASMIDS | ||
| pET-32a-fur | For the expression of P. multocida Fur | This work |
| pSS664 | Asd+ Ptrc pSC101 origin replication p15a, asd+, specr | Derived from pYA3337 |
| pYA4807 | Deletion of fur gene of Salmonella | (6) |
| pSS908 | Insertion of fur into pSS664 | This work |
| pYA4278 | pRE112 derivative, sacB mobRP4 R6K ori Cm+ | (23) |
| pSS909 | pYA4278-Δfur | This work |
| pSS910 | pYA4278-Δfur::kanR, for deletion of fur in P. multocida | This work |
| pMC-Express | Broad-host-range shuttle vector derived from pMIDG100, chloramphenicolr | (24) |
| pSS911 | Insertion of fur into pMC-Express | This work |