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. 2019 Feb 4;13:11. doi: 10.3389/fncel.2019.00011

Figure 3.

Figure 3

Representative pictures of cryostat sections through the DRG from naïve (A,G), sham-operated (B,H) rats and rats with unilateral SNC (C,D,I,J) or CSNT (E,F,K,L) for 7 days. The sections of ipsilateral (C,E) and contralateral (D,F) DRG of the L4 spinal segment as well as ipsilateral (I,K) and contralateral (J,L) DRG of the C7 spinal segment were incubated under the same conditions with rabbit polyclonal antibody recognizing superior cervical ganglion-10 (SCG-10). Scale bars = 75 μm. (M) Graph illustrating the mean intensity of SCG-10 immunofluorescence measured in individual DRG neuron-size classes of cervical (C6-C8) and lumbar (L4-L5) spinal segments ipsilateral (i) and contralateral (c) to unilateral SNC and transection (CSNT) for 7 days; n = 6 for each group. Neurons A ≥ 40 μm; Neurons B 25–40 μm; Neurons C ≤ 25 μm. *Significant difference (p < 0.05) compared to naive or sham-operated rats in a Mann-Whitney U-test.