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. 2019 Feb 4;13:44. doi: 10.3389/fnins.2019.00044

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Copyright © 2019 Dong, Ye, Zhong, Xu, Qiu, Wang, Shao and Xing.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FOXO3 plays a vital role in neuronal apoptosis. SH-SY5Y cells were transfected with pcDNA3.1-flag or pcDNA3.1-Tat-flag only for 48 h, or were treated with scramble-siRNA or FOXO3-siRNA for 24 h followed by transfection with pcDNA3.1-Tat-flag for 48 h. (A) Expression of FOXO3 was analyzed by Western blotting. Statistical analysis is on the right (^∗∗P < 0.01). (B) Expression of nuclear FOXO3 was analyzed by Western blotting. Statistical analysis is on the right (^∗∗P < 0.01; ^∗∗∗P < 0.001). (C) Expression of cytoplasmic FOXO3 was analyzed by Western blotting. Statistical analysis is on the right (^∗P < 0.05). (D) Expression of total FOXO3 was analyzed by Western blotting. Statistical analysis is on the right (^∗∗P < 0.01). (E) Expression of Bim and Bcl-2 was analyzed by Western blotting. Statistical analysis is on the right (^∗∗P < 0.01). (F) Apoptosis of SH-SY5Y cells was detected by measuring caspase 3/7 activity, and the statistical analysis is presented (^∗∗P < 0.01; ^∗∗∗P < 0.001). Statistical analyses of all Western blots were of at least three independent repeated experiments. Vehicle: pcDNA3.1-flag; Tat: pcDNA3.1-Tat-flag. Scramble: scramble-siRNA. siFOXO3: FOXO3-siRNA.