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. 2019 Feb 4;10:46. doi: 10.3389/fpls.2019.00046

FIGURE 8.

FIGURE 8

Changes to phytohormone contents during leaf senescence of two genotypes of Brassica napus L. supplied with high (HN) or low (LN) nitrate for 23 days. Phytohormones (A: MeJA, methyl jasmonate; B: JA, jasmonic acid; C: IAA, indole acetic acid; D: ABA, abscisic acid; E: IP, isopentenyladenine; F: IPR, isopentenyladenosine and G: SA, salicylic acid) were extracted in a methanol/formic acid buffer after 0 and 23 days of HN (3.75 mM NO3-) or LN (0.375 mM NO3-) for two genotypes of Brassica napus L. (Ténor and Samouraï) and measured by UPLC-MS (for details see section “Materials and Methods”). The ratio of phytohormone contents (SA+ABA)/(IP+IPR) was calculated at D0 and D23 (H). Vertical bars indicate ± SD of the mean of three biological replicates. Statistical differences are represented by letters (P < 0.05, ANOVA, Newman–Keuls test).