Figure 4.

Expression of the TCR and accessory molecules CD4 and CD8. (A–C) Flow cytometric of MART1-TCR level by mean fluorescence intensity (MFI) of tetramer staining in Tet⁺ CD8⁺ T cells (A) and Tet⁺ CD4⁺ T cells (B) comparing Control vs. Peptide groups, and in Control group (C), comparing CD8⁺ and CD4⁺ Tet⁺ T cells at different time points after reconstitution (mean ± SEM, peptide n = 8, control n = 9). (D–G) Tetramer staining MFI of Tet⁺ T cells from PBMCs (D), thymic graft (E), spleen (F), and (lymph nodes (G) at end point (mean ± SEM, peptide n = 8, control n = 8). (H) CD8 expression (MFI) on single and double-positive Tet⁺ and Tet⁻ CD8⁺ T cells in thymic graft. (I) CD4 expression (MFI) on single and double-positive Tet⁺ and Tet⁻ CD4⁺ T cells in thymic graft. All panels: filled and open circles depict mice from Peptide and Control groups, respectively.