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. 2019 Feb 4;10:63. doi: 10.3389/fimmu.2019.00063

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Copyright © 2019 Li, Teteloshvili, Tan, Rao, Han, Yang and Creusot.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotype of MART1-reactive T cells in the presence or absence of antigen. (A) Representative flow cytometry plots of CCR7 vs. PD-1 staining on Tet⁺ and Tet⁻ cells among DP, CD8SP, and CD4SP thymocytes from Control group (upper row) and Peptide group (lower row) at end point. (B,C) Percentage of CCR7⁺, CD45RA⁺, PD-1⁺ and TIM-3⁺ cells among Tet⁺ CD8⁺ and Tet⁻ CD8⁺ T cells from Control group (open circles) and Peptide group (filled circles) in thymic graft (B), PBMCs, spleen, and lymph nodes (C) at end point (mean± SEM, n = 8 mice per group).