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. 2019 Feb 4;10:115. doi: 10.3389/fimmu.2019.00115

Figure 3.

Figure 3

Th17-lineage cells are reduced following the inhibition of fatty acid synthesis. Memory CD45RO+CD4+ T cells were isolated from PBMC of healthy donors, stimulated with anti-CD3 and irrAPC for 5 d and stained for CD4, CD161, and ACC1/2. Representative histograms and MFI of ACC1/2 expression on CD4+CD161+ (CD161+) and CD4+CD161− (CD161−) cells (n = 5) (A). Memory CD45RO+CD4+ T cells were cultured for 5 d with anti-CD3 and irrAPC in the presence or absence of TOFA, C75, or cerulenin. Cells were re-stimulated with PMA and ionomycin in the presence of brefeldin A; stained for CD161, IL-17, IFN-γ, CD3, and CD8 and analyzed by flow cytometry (gated on live, single CD3+CD8− cells). The effect of TOFA (n = 4) (B), C75 (n = 8) (C), or cerulenin (n = 9–10) (D) on the frequencies of CD4+ T cells expressing CD161, IL-17 and IFN-γ are shown. (B) *p < 0.05, **p < 0.01.