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. 2019 Feb 4;10:59. doi: 10.3389/fphar.2019.00059

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Copyright © 2019 Palikhe, Ohashi, Sakamoto, Hattori, Kawakami, Andoh, Yamazaki and Hattori.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in protein expression levels of iNOS and total and phosphorylation levels of STAT1 and STAT3 in LPS-stimulated MG6 cells. (A) Typical Western blots of iNOS, phospho-STAT1 at Tyr-701, total STAT-1, phospho-STAT3 at Tyr-705, and total STAT-3 after challenge with 100 ng/ml LPS. GAPDH served as loading control. (B) Time course of changes in iNOS protein expression after LPS application. (C) Time course of changes in STAT1 phosphorylation after LPS application. (D) Time course of changes in STAT3 phosphorylation after LPS application. The results represent the mean ± SEM for three independent experiments. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 vs. time 0 by t-test.