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. 2019 Feb 4;10:59. doi: 10.3389/fphar.2019.00059

FIGURE 2.

FIGURE 2

Effects of STAT1 siRNA transfection and stattic on protein expression levels of iNOS and total and phosphorylation levels of STAT1 in LPS-stimulated MG6 cells. (A) iNOS, phospho-STAT1 at Tyr-701, and total STAT1 before and 15 h after 100 ng/ml LPS application in the presence of or STAT1 siRNAs (siSTAT1) or the negative control siRNAs (siCTL). (B) Concentration-dependent effect of stattic (0.5–2 μM) on iNOS expression before and 15 h after 100 ng/ml LPS application. (C) Effects of siSTAT1 alone, stattic (2 μM) alone, and combination of siSTAT1 and stattic (1 μM) on iNOS, phosphorylated STAT1, and total STAT1 before and 15 h after 100 ng/ml LPS application. (D) Effect of STAT3 siRNAs (siSTAT3) on iNOS and STAT3 expression before and 15 h after 100 ng/ml LPS application. GAPDH served as loading control. Shown are representative Western blots from three independent experiments in which the same results were obtained.